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Leader bleb and cell body area were measured using images of soluble EGFP for non-targeting and hEps8 siRNA treated cells and Emerald tagged to Eps8 in rescue and over-expression experiments by outlining cells in each frame using Fiji (http://fiji.sc/Fiji).sc/Fiji
The appearance suggests dark nonstained white matter pathways known to cross the pons and pockets of intense AQP4 immunoreactivity outlining cells (likely pontine reticular formation nuclei).
Similar(58)
Although a clear differentiation of cell surface from intracellular localizations was not possible, an enforced fluorescence with granular pattern outlining cell shape was observed in all TNF-stimulated cells.
Yellow-dashed shapes outline cells that express very low levels of vim-3 that also express rootletin.
Cell area was quantified from manually outlined cells in digitized microscopic images (recorded by an Olympus Invert scope microscope; Olympus, Tokyo, Japan) of randomly chosen cell fields using the Image-Pro Plus 6.0 software (Media Cybernetics, Silver Spring, MD).
V3FL outlined cell membranes (Figure S1B), whereas V2FL was detected within the cell.
From the immense number of functions of the DE genes, five categories were outlined: cell death, cell growth, metabolism, development and RNA processing.
Aβ1 42 staining appears to outline cell somas.
In order to examine the morphology of neurons differentiated for longer periods of time, we transfected cells with eGFP to outline cell morphology.
Nuclear levels of APPL1 presented as average pixel intensity reflect integrated pixel intensity divided by the area of an outlined cell nucleus region.
The entire cell area was assessed after outlining the cell contours based on the Alexa Flour 546-phalloidin staining.
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