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Although detection limitations are outlined as per diagnostic criteria within the manufacturers' instructions, this study considered a theoretical L.O.D. for each test, based on elevated negative or aberrant results.
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The bacterial suspensions were prepared by 0.5 McFarland bacterial suspension method briefly outlined as follows: 108 colony-forming units per milliliter (CFU mL−1) was diluted to 105 (CFU mL−1) in DifcoTM nutrient broth solution.
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For each axial section, the cell outline was determined based on absence of dextran-AlexaFluor647 staining, and the integrated fluorescence intensities inside the cell outline as well as those of 1 3 independent background regions per section were determined.
Where possible, kidney position will be identified on the localisation images for comparison with planned kidney outlines, as per standard departmental practice.
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