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The following is a brief outline of the protocol used to deplete fibroblasts followed by positive selection of epithelial cells: Resuspend cells at 10/ml in PBS.
We published an outline of the protocol 26 in the PROSPERO International Prospective Register of Systematic Reviews in 2014, registration number: CRD42014012888.
The SOCceR study has established an Independent Data Safety Monitoring Board (DSMB) comprising of independent experts who have no conflict of interest and agree with the outline of the protocol.
The following is a brief outline of the protocol used to deplete fibroblasts followed by positive selection of epithelial cells: To avoid cell clumps, place petri dishes containing cell suspension at ~30-45 ~30-45 angle.
Staged PISs have been designed with information given at the time of registration limited to that which is necessary for consent for release of tumour blocks, plus a minimal outline of the protocol so as to avoid information overload.
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The authors conclude by discussing the various reasons why they think that the explicit, precise, procedurally based CTA approach had value in this instructional context (learning about processes of scientific inquiry), and provide outlines of the protocols used in the CTA videos that could be informative to instructors considering adoption of the approach.
The outline of the experimental protocol is summarized in Figure 1.
The outline of the study protocol is shown in Figure 1.
An outline of the study protocol is shown in Figure 1.
The schematic outline of the experimental protocol is given in Figure 1.
A descriptive outline of the core protocol is provided with some detail in Table 1.
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