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However, no fungal outgrowth was detected in larval cadavers.
Assessment of neurite outgrowth was evaluated by counting about 30 to 50 cells per condition.
After 72 h incubation, the neurite outgrowth was assessed under a phase contrast microscope.
Neurite outgrowth was significantly improved when the polymer containing both neurotrophins was electrically stimulated.
Whole-mounted DRGs were then positioned close to the patterns and neurite outgrowth was monitored.
In contrast, no bias in directional outgrowth was observed for neurites growing on unorganized monolayers.
The DRG cell outgrowth was highly correlated with the density of different hydrogel compositions.
The results demonstrated that neurite outgrowth was highly dependent on the particular type of adhesive protein.
Without NGF supplementation, neurite outgrowth was unaffected by L1CAM blocking or the depletion of Schwann cells.
At 24 and 48 h post-transfection, significant neurite outgrowth was observed in the miR-124a-transfected group (Fig. 2a).
Increase in PDL concentrations did not alter cell survival in 2D cultures but neurite outgrowth was significantly inhibited.
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