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(A) Experimental design for outgrowth analysis.
For neurite outgrowth analysis, about 25 fields of view were automatically captured using a motorized stage.
To determine if the deletion of Cdk2ap1 has any effect on hatching process of blastocysts, we have performed blastocyst outgrowth analysis.
For neurite outgrowth analysis (Fig. 1E), average cell neurite length was measured on the multiple images using the neurite outgrowth plugin.
Statistical analysis was performed using two-tailed t-test except for total neurite outgrowth analysis, for which one-way ANOVA test was used to determine statistical differences between plain and the line substrates with different spacings.
The neurite outgrowth analysis revealed that neurite promotion by the curcumin in the wild-type cells disappeared when the SMN was knocked down.
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This methodology permitted the accurate detection of fine branches extending from thick processes (branch analysis) and determination of the total number of cellular extensions generated by individual cells and all the cells in the images (total outgrowth).
To compare sensitivity of inner cell mass (ICM) outgrowth assay and analysis of culture media amino acid turnover with the sensitivity of the human sperm motility assay (HSMA) and murine embryo assay (MEA) for detection of formaldehyde toxicity.
Thus, to better understand how the presence of the C-terminal tyrosine on α-tubulin influences neurite outgrowth, a deeper analysis of the intracellular organization of axons during outgrowth was necessary.
This ensured that the maximum number of cells per fat pad would be transplanted in order to guarantee successful outgrowths for subsequent analysis.
Although sAPPα stimulates neurite outgrowth, a detailed morphometric analysis has never been done.
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