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To avoid the inter-patient variability, we chose to study the differential gene expression patterns observed in two cell types in culture: hIPCs, pancreatic precursor cells that outgrow from cultured human islets of Langerhans of healthy cadaveric donors, and PANC-1 cells, an established line of human PAC.
Single spheres from day 21 to 26 were plated into 6 well culture dishes and cells were allowed to adhere and outgrow from the spheres in SKP medium for 48 hours.
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Sentiment analysis has various sub streams including emotion analysis, trend analysis, and bias analysis etc. Its applications has outgrown from business to social, political and geographical domains.
The whole plant was characterized with the stem length (distance from the soil to the highest collar) of main tiller and tiller 4 (the tiller that outgrew from the bud of phytomer 4 on the main stem), the number of leaves produced by the main tiller, the total green and dead (only for greenhouse experiment) leaf number, and the green tiller number.
During this time frame, fibroblasts outgrew from tissue fragments and proliferated over the well surface.
EC were outgrown from aortas explanted from CAV-1−/− or WT littermates.
In addition to DNA extracted from HUVECs, DNA from EPCs outgrown from a healthy control were studied for VH rearrangement as negative controls for these experiments.
To underline that message, the macaroni campaign carries the playful theme, "You know you love it," and continues in that vein with lines like "The most fun you can have with your stove on," "Outgrow outgrowing it," "Imported from your childhood" and "Parents need warm cheesy hugs".
Tagged lobsters recaptured from GI (baited) outgrew those from MI (seasonally bait-free) by 14.8% (treatment: F1,96 = 11.7, p = 0.0009).
Moreover, lobsters from GI in 2004 outgrew those from MI in 2002 by ∼10% even though the water temperature at MI in 2002 was warmer than the mean temperature recorded at the buoy nearest to the GI site in 2004 by 0.5 1.2°C at 2 20 m depth.
When planted in white sand and protected from herbivores, seedlings originating from clay outgrew those originating from the nutrient-poor sand, but in the presence of herbivores the seedlings originating from white sand performed better, likely due to their higher levels of constitutive carbon-based defenses.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com