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Briefly, the translocon-associated α-glucosidase I removes the outermost glucose residue as soon as the oligosaccharide is covalently attached to the polypeptide chain emerging in the ER lumen.
The α1-2 glinkageic linkage between the outermost glucose residues ('M'–'N', Fig. 1) from the N-glycan of the nascent glycoproteins is cleaved almost immediately by the membrane-bound enzyme α-glucosidase I (GS-I), forming Glc2Man9GlcNAc2 (Hubbard and Robbins, 1979).
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