Mean stiffness of the Inner, Intermediate and Outer samples was 4.3, 5.4 and 7.2 GPa, respectively.
Then the 'leave-one-out' cross-validation (LOO-CV) and an outer test set including 18 outer samples were used to validate the QSAR model.
They concluded that perfusion was highest in the maternal-side inner samples and the lowest in the fetal-side outer samples.
We did not see any obvious differences in the histology or cell type composition between the inner and outer samples, or by quadrant.
A laser ablation setup including outer chamber, sample tube, sample holder and transport tubing was modelled and optimized using advanced computational fluid dynamics techniques.
Three samples were collected via laser capture microdissection (LCM) to compare directly to whole anthers of the same stage: the outer somatic sample contains EPI plus EN cells (EPI/EN), the inner somatic sample contains two layers (ML/TAP), and the AR cells were dissected as the third sample.
The 0 outer handle sample showed little protection with 30.4 ± 2.3% DNA remaining, whereas 84.6 ± 7.2% remained in the 48 outer handle sample, clearly showing that the envelope provides protection against nuclease digestion.
After thawing the subcutaneous adipose tissue at the connective tissue border was separated into an inner and outer layer sample.
The inner zone control samples stained more strongly for proteoglycans than the outer zone samples, reflecting the intrinsic composition of the meniscal tissue [ 14].
Three of the 5 inner medulla samples cluster with papillary tips, while the other two clustered with the outer medulla samples.
