Outcome of binding best measured downstream Cell target (T) (L_{text{out}}xrightarrow{text{cell uptake}},L_{text{in}} xrightarrow{text{metabolism?}}frac{text{Organelle uptake}}{text{Other binding}} to L + Tmathop{rightleftarrows}limits_{k_{text{off}}}^{k_{text{on}}}{text{LT}}) Concentration at target site unknown Major effects often easy to assess (e.g., cell death) but mechanism can be obscure.
An identical concentration of Beclin 1 ECD (10 μM) was incubated with these liposomes (1.2 mg/ml); the outcome of binding was determined by ultracentrifugation analysis.
The fingers-closing transition (detected by a fluorescence resonance energy transfer-based assay) is the unique outcome of binding a correct incoming nucleotide, both complementary to the templating base and with a deoxyribose (rather than ribose) sugar structure.
Such a mechanism could be one explanation for how the availability of different binding partners regulates the outcomes of multiple binding events involving disordered proteins in a cellular context.
This essay will focus on transcriptional activation as one of the key functional outcomes of TF binding.
By the same token, the new data could provide the basis for developing such B-cell epitope prediction tools via machine-learning techniques (e.g., using artificial neural networks and support vector machines) with emphasis on predicting biological outcomes of antibody binding as continuous rather than dichotomous variables.
We now report that p53 protein is transcriptionally activated in NGF-mediated neuronal differentiation and describe its binding to a number of novel and previously unreported genomic regions as well as the transcriptional outcome of this binding.
A significant outcome of calcium binding is that concurrent with activation, TG2 undergoes an extraordinary conformational change that results in an extended structure [7].
Given a finite number of transcription factor molecules available inside a nucleus [11], an inevitable outcome of their binding to target gene enhancers is an enrichment of these molecules at the locations of their target genes [23].
PDZ selectivity, which we for simplicity define here as the final and reproducible outcome of target binding in the cell in the presence of other potential ligands, may thus still be relatively weak on the genomic level, particularly given the already extensive protein-domain promiscuity and the micromolar specificity of the domains described to date.
