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To explore the effects of parameter settings on the outcome of assemblies, we ran de novo assemblies with a range of minimum match percentages (85%, 90% and 95%), match lengths (19, 23, and 25 bp), and gap penalties (30 and 50) (Table 1).
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In this work, we explore the possibility of controlling the outcome of assembly by scaffolding protein engineering.
The progress and outcome of assembly was monitored by time-resolved X-ray scattering, which allowed us to distinguish between closed shells and incomplete assembly intermediates.
Apparently, genome complexity as well as existing genome resources can influence the outcomes of assembly of sequences generated from next generation sequencing.
We reassembled the D. tertiolecta transcriptome using Newbler v2.5 and the outcomes of assembly in comparison with those obtain from v2.3 are presented as the Additional file 1.
Applying iterative changes of different MIRA assembly parameters revealed that mainly hss (hash saving step) and bph (bases per hash) affected the outcome of the assemblies.
The fact that different sequence sets from the same BAC - although both being representative for the sequenced clone - required different parameters for yielding a 'best' assembly, suggested that experimental and stochastic conditions like fragmentation profile, library representativeness and sequence length distribution influenced the outcome of the assemblies.
Furthermore, it cannot be ruled out that the sequential order of individual reads fed into the assembly controlled the final outcome of the assembly.
We recommend using the multi-fasta file for assembly assessment and variation discovery, since this reflects the most accurate outcome of the assembly process.
A critical outcome of inflammasome assembly is the activation of the cysteine protease caspase-1, which activates the pro-inflammatory cytokine precursors pro-IL-1β and pro-IL-18.
Our studies reveal unexpected heterodimerization interactions between caspase-1 and additional inflammatory caspases, presenting a new outcome of inflammasome assembly.
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