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Next, each of the 370 semi-synthetic outbreaks was supplied as input to the BARD detector and its average performance over the non-commuting outbreak group was compared with its average performance over the commuting outbreak group.
Evolutionary relationships of ISAV revealed the 2007 Chilean outbreak group as a monophyletic clade for f that has a sister relationship to the Norwegian isolates.
Moreover, the sequence from a virus isolated in Nigeria in June 2005 was identical to the sequences in the Kenya outbreak group.
For most patients (n = 240), the LR value was higher than 10, which represents a very strong support for their individual assignment to the outbreak group.
As discussed previously, not all the sequences derived from patients considered to be in the outbreak group were included in the monophyletic group defined by the sequences derived from the PS.
In the eastern outbreak group, the relative direction test resulted in a vector of 27.66 degrees, or an east-northeast direction, with a magnitude of 0.1704 (p-value 0.0020) and the following and adjacent matrices had low magnitude southeast vectors.
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Below freezing temperatures, as low as −15.6°C, were recorded for four of the seven outbreak groups (I, III, IV and V); above freezing temperatures were recorded for the two other groups.
The lowest values corresponded to the patients whose sequences, although included in the outbreak, were the closest to the control and non-outbreak group.
The latter initiative is important for two reasons: first, to ensure a smooth transition from current epidemic response strategies to a preventive approach and, second, to respond to the threat of non-group A meningococcus meningitis outbreaks (group C or W135).
The genotype I viruses from the 1987 , 1997and 2004 outbreaks grouped into clade Ia, Ib and Ic, respectively in a ladder-like topology suggesting temporal clade replacement occurred in each of the major DENV-1 outbreak.
In particular, MVLA-4 could distinguish between epidemiologically unlinked strains that were identical by MLST and PFGE (groups I and II; Figure 2), while isolates from confirmed point-source outbreaks (groups III and IV; Figure 2) were either identical or closely clustered.
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