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When samples with low copy number are used, the full reaction time of 16 hours is needed for the reaction to run out of reagents or to reach a point where the level of non-used random primers is low enough to not interfere with the downstream real-time PCR.
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And when you unexpectedly run out of some reagent, there is, more often than not, somebody in the institute who is willing to share some, even if it's late at night or on weekends.
Due to a transient stock-out of CD4 reagents in one of the laboratories, only 19 ST samples were tested on Day 14.
Due to a transient stock-out of CD4 reagents in one of the laboratories, only 19 ST samples were tested on Day 14. CD4+ T-cell absolute counts were generated at Day 14 but the BD FACSCount flow cytometer did not produce CD4% results.
Extraction controls (n = 10) using molecular-grade water (AccuGENE molecular biology water; Lonza) were used to rule out B. pseudomallei contamination of reagents and equipment (27).
Dendrimer made in this fashion tends to have fewer missing branches, and synthetic procedures can generally be carried out with stoichiometric amounts of reagents, as opposed to the vast excesses frequently necessary for divergent syntheses.
Most of the investigations where immunohistochemistry was used to determine the level and distribution of PR expression in the normal human breast were carried out before reagents capable of distinguishing the two isoforms became available.
The experimental tests were carried out at room temperature with various combinations of reagents.
Loss-of-function experiments were carried out with reagents that are currently available.
Reverse transcription of RNA was carried out using reagents from Fermentas (Cat. No. K1611) according to the manufacturers instructions, followed by PCR.
The degassing of reagents prior to measurements is carried out on a simple degassing unit using the vacuum rectification method.
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