Interestingly, Wnt3a treatment – or CA- β-catenin expression – reduced notch intracellular domain (NICD) levels, a well-known read-out of Notch activation.
We show that the expression of this reporter mimics endogenous Notch activity in the neuroepithelium, providing a faithful read-out of Notch signalling.
This expression is induced by ectopic Notch signalling and abolished when endogenous Notch signalling is quenched, thereby confirming that the pHes5-VNP reporter provides an accurate read-out of Notch activation.
In this study we describe the development and validation of a new Notch signalling reporter and demonstrate that this provides a faithful read-out of Notch activity onset in neural progenitors.
The rigorous validation experiments described above establish that the Hes5-1 provides provides a highly accurate read-out of Notch signalling activity, and that the fluorescent VNP protein is a suitable reporter of promoter activation.
Second, combining different datasets in overlaid kymographs has yielded insight into how the oscillations of full-length Dll1 and Notch1 proteins, activated NICD and a read-out of Notch signalling (nascent Lfng mRNA) are co-regulated along the rostro-caudal PSM axis.
The former provides the solution of the corresponding plane notch problem, the latter provides the solution of the corresponding out-of-plane shear notch problem.
This paper provides a comprehensive evaluation of nonlinear stress fields in the neighbourhood of out-of-plane loaded notches with different opening angles, with the effect of varying notch root radius being included.
Matthew d'Ancona At a time when publishing is tightening its belt so fast that it's running out of notches, the award of a new £10,000 book prize on Tuesday offered a rare splash of new cash, courtesy of the sponsors, Blackwell bookshops.
This study deals with the behavior of short cracks growing out of notches.
