The fluorescent region expressing GFP (and thus Crym-HA) was dissected out for mRNA expression analysis.
To check for the expression of other genes, reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of transgenic plants was carried out for each expression unit on the T-DNA.
"Public interest cries out for free expression in our schools," said the judge, John Grady of Federal District Court, who ruled against the woman, Marina androso-Levato, and her 12-year-old son, a student at the school.
Chemical transformation was carried out for heterologous expression of pET28b-kIspS-C-term in BL21 (DE3) (Mamiatis et al. 1985).
Right ventricle (including the piece cut out for gene expression analysis) and left ventricle was weighed separately.
The same comparison has been carried out for maximum expression (see Methods).
Therefore, all P. pastoris transformants, resistant to 1.0, 2.0, and 4.0 mg mL-1 of G418, were picked out for flask expression tests.
RNA samples from days 0, 3, 7, and 10 were transcribed into cDNA; RNA analysis was carried out for gene expression of aggrecan, IL-1, BMP-7, bFGF, and IGF-1.
Watch out for facial expressions.
OP and AS carried out data mining for expression profiles throughout the life cycle.
