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After repeated out-crossing, (F6) tardbp fh301/+ heterozygotes were selected and in-crossed to generate homozygous tardbp mutants (tardbp fh301/fh301).
Breeds known to have been derived from Abyssinian crosses such as the Ocicat and novel breed development or out-crossing programs using Bengals or other affected breeds should test as well.
In the analysis of structures subjected to stationary stochastic load processes the mean out-crossing rate plays an important role as it can be used to determine the extreme value distribution of any response, usually assuming that the sequence of mean out-crossings can be modelled as a Poisson process.
For dynamic responses we usually seek the mean out-crossing rate into the unsafe region.
In particular, reliabilities will be determined by the out-crossing approach in the context of FORM.
However, the FORM analysis only gives an approximation to the mean out-crossing rate.
This approach is based on a computationally efficient approximation to the conditional out-crossing rate for higher-dimensional vectors.
For the reliability-optimal design, the effect of the spatial and temporal correlation of multiple out-crossing events is investigated.
The mean out-crossing rate thus obtained can then afterwards be scaled down to its actual value.
The paper also offers a simple method for computing the mean out-crossing rate of a response process.
An alternative approach to direct application of the out-crossing rate for Gaussian and non-Gaussian vector processes is considered.
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