Sentence examples for our vesicle from inspiring English sources

We had previously established the homogeneity of our vesicle preparations (Kyoung et al., 2011).

Accordingly, our vesicle budding assay revealed no defects associated with capture into ER-derived vesicles in sys1-Δ0, arl3-Δ0, mak3-Δ0, and mak10-Δ0 strains.

Based on these and the results of our vesicle incubation studies demonstrating that calsyntenin-1 is cleaved in syntaxin13 immunoisolates, but not in Rab11 and APP immunoisolates, we concluded that calsyntenin-1 is cleaved in early endosomes.

Using a negative staining protocol, we determined that the average diameter of our vesicle preparations at a lipid-to-receptor ratio of 1000 1 was 83 nm±12 nm.

DOI: http://dx.doi.org/10.7554/eLife.05531.008 Next, we set out to fit HS-induced responses with our vesicle state model to assess synaptic release parameters including RRP, and rate constants for priming, unpriming, and fusion.

By ignoring vesicle replenishment during HS-stimulation and the delayed onset of the HS-induced response, our vesicle state model can be simplified such that an analytical solution can be obtained that qualitatively captures the main features of HS-induced release.

We compared the efficiency of RNA intracellular delivery of our vesicles with Lipofectamine, a commercially available liposome transfection system.

Although β1 could not be identified in our vesicles, β1 is found in rat liver early endosomes [ 42].

Although immunoblotting and mass spectrometry indicate that neuronal clathrin light chain is absent from our synaptic vesicle preparation (Figure 1), we could not definitively exclude that our synaptic vesicle fraction actually contained a small number of endocytic vesicles, which had merely lost their coats during purification [28].

We decided to reanalyze our synaptic vesicle preparation, in an attempt to find these "missing" proteins, using a new, highly sensitive Orbitrap® machine [22].

Such a complex may be regulated by the action of the protein Reticulon 3, which was also found in our synaptic vesicle fraction (data not shown), and is known to inhibit the activity of BACE [57].