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Our titration of the suspension used by Tigertt et al. is based on the assumption that the two strains are equally infectious in guinea pigs.
Our titration of crude cell extracts of cells in which expression levels of N-S-β were induced with 0.01, 0.02, or 0.05 mM ara revealed 1 Y per β and 2 Fe atoms per β!
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We showed that heat treatment decreased infectious MERS-CoV below the detection limit of our titration assay, and this might function as a relatively easy and cost-effective measure to prevent transmission.
In our hands, direct titration of p53 15-31Flu with hDM2 resulted in the expected increase in fluorescence anisotropy and a dissociation constant of 74 nM was determined based on a 1 : 1 isotherm (Fig. S2, ESI †).
The cytokine concentrations and combination were based on published studies in which IFN-γ potentiated T-cell recruitment induced by TNF-α 13, 15, our own titrations of TNF-α in adhesion studies (e.g. 14) and preliminary studies, which verified that combination of TNF-α and IFN-γ was more effective for inducing PBL adhesion then either alone.
When the two effects were combined, only weak binding could be observed over the range of our titrations, indicating a Kd value greater than 4 μM.
Our observations indicate that titration of β-oxidation impacts TCA cycle dynamics; that is, production and/or catabolism as well as efflux of TCA cycle intermediates (TCAi).
The analytical sensitivity (titration) of our test was comparable to the diagnostic sensitivity (clinical samples) better than, the real-time PCR or nested PCR.
Our previous reported potentiometric titration of DHCC shows a single observable midpoint potential, which is consistent with the conclusion that the two heme-binding pockets have similar chemical environments.
Independent synthesis and titration of our original HTS hits showed that the two epimeric alcohols 1 and 28 had similar potency (GI50 = 120 and 62 nM, respectively), while desmethyl ethanol derivative 30 lost some activity (GI50 = 630 nM).
In our sample the dose or titration of pregabalin did not seem to be related to the occurrence of side effects, which occurred in only six (13%) patients assuming ≤150 mg/day.
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