Sentence examples for our pla from inspiring English sources
Our PLA results indicate that ROQUIN and AMPKα1 endogenous proteins in primary C57BL/6 mouse embryonic fibroblasts (MEFs) are close binding partners (new Figure 3c, d).
Overall, our PLA clearly corroborated immunoelectron microscopy and TR-FRET results, thus undoubtedly demonstrating that D2R-A2AR oligomer formation was reduced in experimental parkinsonism.
The computerised data displays created from our PLA Options Assessments enabled us to readily share information at subsequent face-to-face meetings or by email.
The latter literature data are consistent with (i) our PLA staining results for pS396-404 Tandand BIN1 and (ii) the fact that 3-repeat Tau isoforms are expressed in primary neuron cultures.
In our PLA experiments, wild-type NMJs showed punctate signal between the adducin-like isoforms of Hts and Dlg at the postsynaptic membrane, thus indicating that these proteins are in close proximity to each other in this region and likely form a comple x (Fig. 3A A″).
To create our PLA probes we conjugate a rabbit anti-CB1R with a PLUS oligonucleotide (Sigma, Duolink® In Situ Probemaker PLUS catalogue number DUO92009) and a rabbit anti-CB2R with a MINUS oligonucleotide (Sigma, Duolink® In Situ Probemaker MINUS catalogue number DUO92010) following the manufacturer's instructions.
Indeed, these findings were confirmed by our PLAs, since no interaction was observed with Thr231-phosphorylated Tau species in primary neurons.
In our research, PLA was also chosen as hydrophobic segment of a block copolymer due to the higher compatibility between the micelle core-forming PLA and the ethaselen molecules.
Our Fab-PLA method could only detect the pentameric but not the monomeric IgM, whereas 1-PLA detected both IgM forms equally well (see new Figure 2).
The reviewers asked us to use additional independent approaches to validate our Fab-PLA experiments and prove that indeed mIg dissociates during BCR activation.
We agree with the referees that our Fab-PLA method cannot distinguish between a complete dissociation or an opening of the BCR and we therefore now use the term "opening" throughout the new version of our manuscript.
