By combining our linkage maps with actual chromosome counts, we found strong evidence for a major chromosomal rearrangement between L. parva and L. goodei.
Genomic synteny was investigated by comparing our linkage map with the threespine stickleback genome (Jones et al. 2012) and the linkage map of North American ninespine sticklebacks (Shapiro et al. 2009).
In addition, intraspecific and interspecific comparative genomic analyses were conducted to assess possible chromosomal rearrangements by comparing our linkage map with that of North American ninespine sticklebacks (Shapiro et al. 2009) and the threespine stickleback genome (Jones et al. 2012).
We sought to compare our linkage map with maps previously published by Gross et al. (2008) and O'Quin et al. (2013) that also examined synteny between Astyanax and Danio.
Participants who entered or exited the study in a calendar year due to consent or loss-to-follow-up contributed a full person-year because we assumed that provincial in- or out-migration would have been trivial, and our record linkage with the PHOL ensures that we would have detected any testing conducted in Ontario in that year.
Using these jurisdictional keys, it was possible to directly compare our linkage quality results with those from each of these jurisdictions.
Our linkage was conducted with the Healthcare Registration File which includes, for all beneficiaries of the universal public health system, information such as birth date, sex, postal code and year of death (if applicable).
Our linkage results, coupled with plausible biological functions of the potential candidate genes related to aging, such as Klotho, PARP4, DOK6, and ASAMTS9, make these genomic regions good candidates for further investigation of causal variants influencing LTL in this minority population.
Our linkage maps roughly agree with the previous estimation, about 1000 cM [ 29].
Our linkage data in combination with mapping of homeologous regions in the salmon genome provide substantial support for the presence of these mechanisms in Atlantic salmon.
