Our lamina-based quantitative imaging method provides a robust and effective method for different cell types and cells expressing mutations in lamina genes.
We limited our definition of lamina propria to the small intestine both because segmentation was clearer and because lamina propria in the large intestine contains additional connective tissue which we captured in the collagen component.
Apoptotic nuclei are rare in normal adult mice GM, and in accordance with the previous studies in adult rat muscle (Allen et al., 1997), our triple labelling (lamina, TUNEL, Hoechst) showed that the majority of apoptotic nuclei were located in ECM in the adult muscle.
TT8 does not yet have a demonstrated role in regulating trichome initiation on the leaf lamina but our mapping results, together with evidence for a role in trichome initiation on leaf margins and expression in the leaf lamina in response to jasmonic acid [ 19], suggests that such a role merits further study.
Since our hypothesis was that lamina shape variation was a product of differential gene regulation arising from interspecific polymorphism(s) within the leaf QTL interval, we narrowed our focus to the genes with eQTL surpassing the significance threshold in this region.
In comparison with these previous methods, the advantage of our method is that the lamina information is preserved in one axis of the three-dimensional cortical map.
Since the outer surface corresponds either to a final lamina or truncation, our dataset should be comparable to others from cross-sections.
Since our data do not allow for discrimination between lamina and nuclear pore association, this might explain our observation in that the up-regulated genes could move toward the nuclear pores.
Our immunocytochemistry thus confirms that the lamina neurons seen in the Cha-Gal4 reporter line actually express ChAT-immunoreactivity.
If the rdl-Gal4 does in fact represent RDL distribution, there must be a differential distribution of GABAA and GABAB receptors in lamina neurons visualized in our study.
Consistent with our previous data [ 28], those lamina propria cells were mainly CD68 positive macrophages.
