The main drawback of our study was the impossibility to use a gold standard assessment to diagnose autonomic function.
One limitation of our study was the impossibility to test the fluorophore genotoxicity in the in vivo cheek pouch cells, as the keratinization process in itself induced DNA degradation [ 21].
However, a problem inherent to the design of our study is the impossibility to discern what genes were expressed by tumour cells and what signal was derived from TILs themselves.
"The changes in our life must come from the impossibility to live other than according to the demands of our conscience not from our mental resolution to try a new form of life," says Leo Tolstoy.
A major limitation we encountered is related to the impossibility to adjust our results to national gross domestic product per capita corrected by local life costs.
On the other hand the not so great number of patients could be surely considered a limit of our study, together with the impossibility to evaluate the measurement variability as explained in "Methods".
In particular, our interpretations are limited by the impossibility to preserve the ancestral parasite strain throughout the time of the experiment.
In our opinion, it results from a relative impossibility to produce stable non-planar nucleobase pairs, so creation of some complex 3D structure out the innate planar elements requires usage of the sophisticated techniques.
Others methods had not been used due to the unavailability of the program despite the indication in the corresponding papers or to impossibility to modify the source code to used our microarrays data.
This lack of detail is due to the small size of this species and to the impossibility to reduce the field of view of our CT scanner to less than 16 cm.
