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As mentioned in the Methods section, our alignments considered only uniquely aligned reads and sites with quality-score Q≥30.

The answer is that while our alignments and statistical tests take into account only uniquely aligned reads and quality scores Q≥30, Trinity does not apply these criteria.

Within our alignments, there are 78 known RNAs.

The results of our alignments revealed one validated SNP that was conserved among highly divergent taxa.

Based on our alignments of complete gene sequences in bats, we identified two highly variable regions (exons 7 and 17).

However, RNAz was able to identify the majority of known RNAs that were contained in our alignments.

To determine to what extent ErbB3 contributes to the divergence of the ErbB family, we ran the BPPS procedure by removing ErbB3 sequences from our alignments.

Our alignments of the transcript-derived models to the genome can lead to the improper placement of the final coding nucleotide associated with the beginning or end of an exon.

Our alignments validated these difficulties in that we found ITS sequences to be highly divergent across a wide taxon sampling, with long alignments and comparatively high percentages of parsimony-informative positions.

Our alignments therefore represent the "ideal cases" of true alignments.

All these contaminant sequences were removed from our alignments.

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