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We also replaced the Charged domain 1 (237–271) by the extended Variable region B (225 289) in order to reflect the sequence variation found in our alignment for this area.
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We then searched our alignments for the corresponding CNEs located near to these genes based on the annotations provided by Kim and Pritchard [ 32] which uses the human genome as a reference point.
Figure 5 Location of the centers of mass of single cross-sections for each projection angle (blue) and the least squares solutions to fit the viable paths (red) given by Equation 3. The results from cross-correlation for two cross-sections are given in (a, c), and the results from our alignment method for the same cross-sections are shown in (b, d).
Next, we further test our alignment methods for refining the density maps of the complexes by averaging over all aligned subtomograms.
For our alignment, protein-coding genes were constrained to align by codon and tRNA-coding genes were constrained to align by regions of potential secondary structure [ 38].
We ran RAxML with the VT substitution model and the gamma model for rate heterogeneity, parameters which are most suitable for our alignment according to ProtTest (Darriba et al. 2011).
Now from Equation 3, if our alignment is good, then for each cross-section x, there should exist some c x so that Θ c x ≈t x.
Herein, the two subsets for our alignment in AVANA were i) 8 MMP-13 sequences and ii) all other MMPs (42 of them).
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