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As a future direction, such advanced hepatocyte culture systems can be connected to other tissue models for evaluating tissue-to-tissue interaction beyond cell-to-cell interaction.
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Considering the mechanism identified in this study, the consequences of hormone-related effects by PFOA should be evaluated in other tissues, models, and sensitive life stages.
We show that tumors in an orthotopic tissue microenvironment grow more robustly and develop vasculature more rapidly than subcutaneous and other ectopic tissue models.
Growing tumor spheroids in different microenvironments revealed that tumors in an orthotopic tissue microenvironment grow more robustly than subcutaneous and other ectopic tissue models.
Arrhythmias can be induced in wedges (and other cardiac tissue models) by special pacing protocols, and several studies have examined how drug actions change the vulnerability of cardiac tissue to the induction of arrhythmias, including ventricular fibrillation (Weiss et al., 1999; Qu and Weiss, 2005; Seigneuric et al., 2005).
We compared our constructed kidney metabolic model with two other tissue-specific models obtained from previous work [ 6, 9], including heart specific model and liver specific model.
This mechanism has been explored in a number of other cell and tissue models with results that are consistent with our proposal.
DeadEasy Caspase could be used for automatic counting of apoptotic cell number in other tissues or model organisms (e.g. fish, mouse) so long as stainings of comparable qualities are used to visualise cells of comparable sizes, as indicated in Table 1 and Table 3 and Figure 4.
If needed to complete critical relationships within the network model, other tissue contexts were also considered, based on our assumption that they would reflect the response to stress in normal lung and cardiovascular tissues.
These objectives are being realized by the National Cancer Institute Clinical Proteomic Tumor Analysis Consortium, which is applying standardized proteome analysis platforms to analyze tumor tissues from the TCGA program as well as unique cell and xenograft models and other tissue collections, all of which are accompanied by rich genomic datasets [ 60].
Studies of miR-122 in other tissues or disease models are extremely limited.
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