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It occurs through accumulation of fluid and other tissue elements that would normally be drained by the lymphatic system.
Muscle tissues are normally displayed with HU values between 50 and 100 HU though within a normal muscle belly there are also other tissue elements such as connective and fat which are coded with much lower HU values.
However, the high correlations measured suggest that the contribution of other tissue elements is relatively small in the regions investigated.
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Any yellow-brown stained endothelial cell or endothelial cell cluster clearly separate from the adjacent microvessels, tumor cells, and other connective tissue elements was considered a single, countable microvessel.
According to an early study (Weidner et al, 1991), any highlighted endothelial cells or cell cluster clearly separate from adjacent microvessels, tumour cells and other connective tissue elements should be regarded as a distinct countable microvessel.
We regarded as a distinct countable microvessel any highlighted endothelial cell or cell cluster clearly separated from adjacent microvessels, tumour cells, normal cells and other connective tissue elements according to the international consensus (Vermeulen et al, 1996).
Any yellow brown stained endothelial cell or endothelial cell cluster that was clearly separate from adjacent microvessels, tumor cells, and other connective tissue elements was considered a single, countable microvessel.
Ten most vascularized areas (hot spot) were selected at low magnification and single red-brown stained endothelial cells, endothelial cell clusters, and microvessels, clearly separated from adjacent microvessels, tumour cells, and other connective tissue elements and MCG/D were counted at ×400 fields in a 0.19 mm area.
Microvessel counting was a modification of the method described by Weidner et al. (1992) in which large microvessels as well as any single stained endothelial cells clearly separated from adjacent microvessels, tumour cells, and other connective tissue elements were considered single, countable microvessels.
Consistent with other tissue-specific elements whose evolution occurs at higher rates,, we have shown that muscle-specific enhancers are strongly- but not ultra-conserved.
Any brown-staining endothelial cell, containing a visible nucleus, and clearly separate from adjacent microvessels, tumour cells and other connective-tissue elements, was considered a single, countable microvessel, without requirement for a lumen or the presence of erythrocytes.
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