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Mouse phenotypes induced by our high fat feeding protocol were similar to those observed in other time course studies of HFD-induced adipose tissue inflammation and insulin resistance [4] [6].
Based on other time course experiments conducted, we anticipated M values of approximately 1.0.
An extended comparison of FBPA with other time course analyses methods can be found in [ 27], where we also describe the performance of FBPA on other real data sets as well as simulated data sets.
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Experimenting with other sets of parameters, we have been able to observe other time courses that might be found in an ailing patient.
For other time courses, linear fits (with and without the time logged) were computed for each remaining barcode.
All the other time courses of immobility not presented in Fig. 6 are shown in supplementary material Figs S1– S1.
Beyond cell culture applications, we predict the bias correction algorithm to be just as useful for other time-course metabolomic data.
In contrast, other time-course studies have failed to detect a change in the distribution of nucleotide analogue label-retaining papillary cells after injury [ 128].
We have shown in other time-course studies that metabolic, body composition, and cellular signaling measurements appear to be quite stable from 4 to 20 weeks of CR; so, given that our focus was on different levels of CR rather than timing of exposure, we thought this was a reasonable strategy.
That other time, of course, will no longer come.
On the other hand, time course experiments showed that ZEB1 accumulation coincided with enhanced expression of COL5A1 and Fibronectin in U87 cells, further suggesting a role of ZEB1 in mesenchymal transdifferentiation (Supplementary Figure 4).
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