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GST pulldown experiments showed that GST-GRD can associate with Rac1a and RacE among other Rac GTPases (data not shown).
Preliminary data from our laboratory demonstrates that Ncad may genetically interact with other Rac GEFs to affect earlier CNS development Ncad mutants cannot be rescued by expression of RacWT alone in the CNS (Biersmith, B. and Geisbrecht, E.; unpublished data).
These neurons never express other Rac proteins.
Two other Rac related proteins, RAC-2 and MIG-2, could, in principle, be additional targets of the CYK-4 GAP domain.
In particular, links to permanent admissions were more likely to be false positives than links to respite admissions or hospital leave, and the sensitivity of the strategy was lower for admissions (under 75%) than for other RAC events (over 85%).
Exhibiting an 89% and 92% identity to Rac1 and Rac2, respectively, Rac3 differs from other Rac proteins only in the carboxyl terminus, a region essential for subcellular localization and regulatory protein binding [ 8, 9].
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Citterio et al. recently reported that other Rac-GEFs in addition to P-REX1 are upregulated in luminal breast cancer.
We chose the Raichu-Rac reporter over other Rac-FRET biosensors as the probe most likely to provide best signal-to-noise ratio without affecting cell survival.
Other RAC-E studies have shown that greater practice nurse involvement in the care of patients with diabetes and obesity is cost-effective [ 19, 20].
Importantly, whereas our findings identify Dock180/Elmo1 downstream of LPA/S1P in MEFs, our experiments cannot rule out involvement of other RAC-GEFs within this pathway.
Significant furrow ingression is not restored by depletion of either RAC-2 or ARX-2 in nop-1; ced-10; cyk-4 R459A embryos, suggesting that the cytokinesis defect is not due to activation of other Rac-family proteins.
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