To facilitate the surveillance of the antigen targets included in Bexsero® (fHbp, PorA, NHBA and NadA) for protective immunity, a Bexsero® Antigen Sequence Type (BAST) scheme, based on deduced peptide sequence variants, was implemented in the PubMLST.org/neisseria database, which includes the MRF-MGL and other isolate collections.
Eight healthy Holstein-Friesian heifers in mid-lactation were experimentally infected using a split-udder design with 3 different CNS strains: one Staphylococcus fleurettii (from sawdust bedding) and 2 Staphylococcus chromogenes strains (one isolate from a teat apex, the other isolate from a chronic intramammary infection).
With both sera, the reactivities were found to be weaker towards the LD antigens of the isolate obtained from the patients themselves than against the other isolate.
STs 6 and 7 are single locus variants and ST5 is a singleton (differing at two or more genes from every other isolate).
Consistent with the location of magA within the cps operon of K1 isolates [52], both K1 groups were magA positive, while magA was not detected in any other isolate.
One isolate, HK/415742/09, was characterized from the first patient with pandemic influenza A(H1N1) 2009 virus infection in Hong Kong in May 2009 [24] and the other isolate was a mutant virus, HK/415742/09-Mut after mouse adaptation of the clinical isolate.
To compute the mean Hamming distance, we compared the sequence of each isolate to that of every other isolate, finding the number of differences between them, and dividing the total number of differences by the number of pairwise comparisons within that period.
The other isolate used for construction of a third-stage dispersal juvenile library was achieved by collecting directly from chips around pupal cells of the vector beetle (Monochamus alternatus) in a dead pine tree in winter from the same site where the Chinese propagative isolate was obtained.
Only 1 (2%) isolate was resistant to chloramphenicol and 1 (2%) other isolate was resistant to clindamycin and erythromycin.
One other isolate, possibly related to CMRSA-2, was recovered from a US veterinarian in small-animal practice.
In 8 of 10 instances the alternate allele was present as the primary allele in at least one other isolate.
