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Fifty samples were positive by the developed assay, whereas 51 samples were positive by two other assays.
Therefore, the RT assay cannot be used interchangeably with the other assays to monitor individual patients.
Other assays perform best with a pre-amplification, high-temperature denaturation step ("hot start").
Other assays.
Examinations of blood were performed only if other assays failed to show trypanosomes.
Also other assays developed in our lab showed equal sensitivities on both machines.
For proliferation and all other assays, treatment was started 1 day after plating.
Availability of numerous other assays makes the assessment much convenient.
The overall agreement between the new assay and two other assays was 98.5%.
MTT and fluorescence tests were performed in esaplicate; all other assays in triplicate.
Relative to the other assays, the Axsym had a higher sensitivity (78.9%) but lower specificity (86.5%).
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