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In the adjusted analysis, patients with RA and OA had the best results in terms of the greatest improvement in OSS, compared to FS patients whose mean change in OSS was 4.4 units less (p = 0.001) (Table 4).
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Fluorescence images of OCN ascertained that gap junction (Cx43) was affected by OSS even in the absence of OM compared to those of the control, suggesting that the shear stress stimulates the cells mechanically and alters cellular functions.
This was confirmed in the migration assay where a statistically significant lower number of cells migrated towards Bio-Oss as compared to MTA and dentin chips.
The fluorescence images presented more deep staining in the cells stimulated by OSS with OM compared to those of control.
Compared to the OSS score, this means a score of 15 points [ 1].
This study highlights that MTA and dentin chips have a greater potential compared to Bio-Oss regarding the attraction of dental stem cells and are good candidates for bioengineered pulp regeneration.
There was a lower degree of floor and ceiling effects in SPADI compared to WORC and OSS.
As shown in Figure 2(a), the material with the highest porosity was the dentin chips, when compared to MTA and Bio-Oss.
Moreover, a statistically significant lower number of cells migrated through the transwell filter when Bio-Oss was added in the migration chamber compared to all the other materials.
The pre-operative and six-month follow-up OSS and CS (including the individual components) were then compared to analyze the difference achieved after ASAD.
Overall, dentin chips favored the migration of cells compared to the other materials, whereas Bio-Oss inhibited the migration of dental mesenchymal stem cells as compared to MTA and dentin chips.
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