At the core of our approach is a small hidden OS-repair-image that is dynamically created from the healthy running OS instance.
This results in improved selectivity toward tumors with diminished levels of O-alkylguanine-DNA alkyltransferase (MGMT), the resistance protein responsible for O-alkylguanine repair.
Thus, 90CE demonstrates high selectivity toward tumors with diminished levels of O-alkylguanine-DNA alkyltransferase (MGMT), the resistance protein responsible for O-alkylguanine repair.
Regardless of skeletal maturity, the defects treated with TEC are completely filled with Safranin O positive repair tissue with good tissue integration.
Strategies were: (N) non-repairers regardless of the damage segregation strategy when the latter had insignificant effects on fitness; (S) symmetric division and (O) optimal repair.
Because of a greater reliance on guanine O-6 alkylation for cytotoxicity, 90CE and its prodrugs exhibit greater selectivity than BCNU toward cells with deficient O-alkylguanine repair in many model systems.
The normal function of AGT is to repair O 6-alkylated guanine in DNA by transferring the alkyl group in an SN2 reaction to a reactive Cys145 residue in AGT.
These genes belonged to three COG categories (L: DNA replication, recombination and repair; O: Posttranslational modification, protein turnover, chaperones and Q: Secondary metabolites biosynthesis, transport and catabolism).
This revealed a bias in the representation of genes showing >1.2-fold increase in expression in response to the addition of OHHL within COG categories C (energy production and conversion), L (DNA replication, recombination and repair), O (posttranslational modification, protein turnover, chaperones) and P (inorganic ion transport and metabolism).
MGMT is an important enzyme that can repair O-alkylguanine adducts on DNA and plays a significant role in the resistance to alkylating agents in GBM [16], [17].
