These ChIP experiments revealed that, for all the three analyzed origins, origin DNA was enriched 2 5 times over control by immunoprecipitating chromatin with antibodies recognizing Rb and E2F1 (Figs. 1B and 1D for Lamin B2 and GMS-CSF origins respectively).
At each of these origins, origin recognition complex (ORC) binding was abolished by introducing a four-base-pair mutation in the ORC-binding site at the native chromosomal loci.
An important consideration to highlight here is that pre-RCs are laid down at potential origins (origin licensing) during late telophase in the mitotic cell cycle (Dimitrova et al. 2002), but lost if cells become quiescent (Madine et al. 2000; Tatsumi et al. 2003; Cook et al. 2004) necessitating their re-synthesis and recruitment following cell cycle re-entry.
15 Origins origins.net Origins is a subscription service for British and Irish records, with substantial data sets from the Society of Genealogists, such as apprenticeship records and wills, and a growing collection of census indexes and images.
One of the reasons I am championing Carnie Holdsworth is because we share class origins; origins that make one only too aware of how hard it is to even realise that one could have a voice through the pen.
Sam 2: SAM synthase 2 gene sequence, His 4 ORF: Histidine 4 open reading frame, pBR322 origin: Origin of replication of the plasmid pPIC9K, lac Z: β-galactosidase gene Z, cbs: Cystathionine-β synthase gene, pUC origin: pUC origin of replication, Zeocin: Zeocin resistance gene.
The frequency and amplitude of spontaneous subthreshold responses were analyzed using the software Origin (Origin Lab Corp. Wellesley Hills, MA, USA).
There was no evidence for origin-environment interaction in plasma carotenoid levels (non-significant term "BSM*origin(duplicate)"), but there was a significant effect of nest of origin ("origin(duplicate)") (Table 2).
Data were analyzed with Origin (Origin Lab, Northampton, MA, USA), Microsoft Excel and Prism (Graphpad) software.
Figures were prepared using the Origin (Origin Lab, Inc., Northampton, MA) or CorelDraw (Corel Corporation, Ottawa, Canada) software packages.
The extracted time traces were processed using Matlab (MathWorks, Natick, MA) and Origin (Origin Lab, Northampton, MA).
