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Similar data were obtained in two independent time-courses and at other replication origins (data not shown).
To search for more cancer-associated TRKB point mutations, we sequenced the exons comprising the TRKB kinase domain from genomic DNA of 28 human tumor cell lines, from several tissue origins (data not shown).
However, these two particular skews and the remaining 254 tetranucleotide combinations failed to reliably predict Thermococcus origins (data not shown).
Using a series of different primers, this genotyping technique identified clonally indistinguishable E. asburiae isolates, although they were from various geographic origins (data not shown).
The great majority of the sequences from diploid wheat and Aegilops species show a clustering according to their genomic origins (data not shown).
Despite the higher sequence diversity of GPCRs in fly, mosquito, C. elegans and other vertebrates, sequence analysis suggests evolutionary conservation of GPCRs across phyla and that they might have ancient origins (data not shown).
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Examination of the data showed that the regression was linear, but the plot did not pass through the origin (data not shown), suggesting that removal of oxamyl on silkworm feces activated carbon involved intraparticle diffusion but not the only rate controlling step.
Examination of field samples revealed considerable variation between samples and no clustering was evident based on nest or location origin (data not shown).
This enhancer was also active in a cell line derived from a human rhabdomyosarcoma, a tumor of muscular origin (data not shown).
Phylogenetic analysis of the entire B. pseudomallei MLST database revealed distinct clustering of Asian isolates (including Malaysian, Singaporean, Philippine and Indonesian STs) separate from those of Australian origin (data not shown).
An explorative neighbor-joining (NJ) tree was constructed in MEGA4, using a maximum composite likelihood substitution model (default settings), to verify that all clones were of patient-specific origin (data not shown).
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