Because mATG9 is a transmembrane protein, it is likely that it is incorporated into autophagosomes precursors with some membrane from its originating compartment.
In this study, transport rates for unphosphorylated STAT5B were determined in single cells by FRAP and found to follow saturation kinetics dependent on both STAT5B-GFP expression level as well as size of the originating compartment.
The results for no normalization, normalization by the nuclear surface area and normalization by the originating compartment volumes are shown in fig. 2B-D, the estimated parameters are shown in tab. 1.
These observations and the high variability in the morphology of fungal extracellular vesicles [14] support the hypothesis that the vesicle populations originate from compartments of distinct biogenesis, which could involve both Golgi- and exosome-derived pathways.
Irrespective of the nature of the primary disease and the originating renal compartment, renal fibrosis is considered to be the common final pathway by which kidney diseases with variable etiology progresses to end-stage renal failure.
A subgroup of higher molecular weight proteins represented in EMVs appear to be post-translationally modified, compared to same proteins in whole-cell extracts, suggesting that they may originate from compartments characterized by high glycosylation, such as BEC luminal membranes or endocytic vesicles(s).
A possible origin of the GFBs from an endosomal or vacuolar compartment would be consistent with the observed insensitivity to BFA and monensin, as a secretion pathway originating from these compartments would be after the Golgi block caused by the drugs.
We interpret the multiple concentration peaks to indicate water masses originating from different compartments in the watershed are reaching the stream at different times and with different chemistries.
These primary analyses resulted in 207 proteins that likely reside in one of the endomembrane compartments originating from the ER.
3,757 SP-containing proteins do not contain a TM domain and are therefore either secreted or reside in various subcellular compartments originating from the ER.
