Populations originated from intact, never restored prairies within a 80 km radius of the reciprocal garden planting site (Table 1).
Therefore, the presence of immunoreactive Aβ in the mouse brain after i.v. injection of Cy5.5-labeled human Aβ peptides suggested that these peptides were blood-borne and confirmed that at least a portion of imaging signal originated from intact Aβ-Cy5.5 conjugates.
The rFab were added at a concentration sufficient to compete binding of the originating intact mAb to GAD65 by at least 80% (0.7 1 μg/ml).
This environment is highly competitive due to the nutrient-rich rhizodeposits consisting of a wide variety of compounds derived from sloughed off root cells and tissues, mucilages, and soluble exudates originating from intact roots [6].
Organoid cultures can originate from intact tissue, such as tissue slices, or from cells cultured to form or resemble 3D cultures.
As expected given our poly-A enrichment strategy, most transcripts in our data originate from intact protein-coding genes, but we also observed four other biotypes represented by more than 100 distinct transcripts.
Because the WT and KO cells had previously been cultivated in monolayer in the presence of serum, the ability of HIF-1α to promote SFE was confirmed using tumor cells derived from freshly digested Hif1a DF, PyMT+ tumors originating in intact transgenic mice.
In these cases, coverage estimates most likely represented duplicate mappings based on transcripts that originated from the intact copy of the gene (Additional file 1: Figure S5).
Selected samples originated from 25 intact female dogs of different breeds, which had undergone surgery, consisting either of a regional or a radical mastectomy, due to mammary gland tumor.
In flowering plants, there is a second class of symplekin gene, typified by the Arabidopsis At5g27595/At5g27590 gene, which seems originated from an intact symplekin gene being split by an intergenic region.
To determine whether measured Cy5.5 fluorescence in imaging experiments originated from the intact Cy5.5-Aβ1-40 conjugathanrather than from the proteolytically-degraded fragments or dye alone, Aβ peptides were detected in the brain tissues of wild-type and Abcg2-KO mice using an anti-Aβ antibody, 6E10.
