One reason pain studies have lagged behind is that many of the techniques originally developed cannot be directly implemented to study key relay sites within pain pathways, such as the skin, dorsal root ganglia, spinal cord, and brainstem.
This method, as originally published, cannot resolve causality, i.e. which gene is the regulator and which gene is the target for a given significant interaction, as it relies solely on the symmetric MI matrix and can not take advantage of the kinetics represented in time series data.
Although the GL-1 cell line does have 25 q LOH, the normal karyotype reported when this line was originally published cannot distinguish whether loss of the wild-type allele and/or amplification of the FLT3 ITD was present initially or developed with continued in vitro propagation.
In some cases, deforestation has led to the vigorous growth of shrubby plants that form a scrubland so dense that the originally dominant trees cannot return.
I cannot originally experience myself as something a voyeur, for instance.
We hypothesized that the shape is more complex than originally believed, and cannot be accurately modeled as a spherical structure.
Similarly, if one is prepared to admit a man with ten thousand hairs on his head is not bald, then one can argue that even with one hair on his head he is not bald since the removal of any one hair from the originally hirsute scalp cannot make the relevant difference.
With no task transfer The tasks that are originally assigned to CPTs cannot be transferred to CPs.
As originally developed QuikChange™ cannot introduce multiple mutations, a modified version of the kit (QuikChange™ Multi Site-Directed Mutagenesis kit) has been released and some other adaptations have been reported [ 14- 19].
