Sentence examples for original mutations from inspiring English sources
Genomes carrying the original mutations in S3 or S4 plus compensatory mutations restoring base pairing were viable and had robust growth phenotypes.
At the base of the tree are the original mutations that triggered the tumour in the first place: mutations that should be shared by all of the cancer cells in the tumour.
The nature of selected revertant genomes might allow conclusions about the underlying mechanisms affected by the original mutations.
Of note, when stable cell lines were generated in the absence of DEB025 or CsA, the mutant replicons retained their original mutations and did not revert back to the WT sequence (data not shown).
The use of ATCC21577 H37Ralsoso minimizes undesired genetic changes unrelated to its original mutations involved in virulence attenuation, which is endorsed by the finding that only 272 genetic variations were identified between H37Ra and H37Rv, a relatively small number of sequence differences compared to 736 SNVs identified between the attenuated strain BCG Pasteur and M. bovis AF2122/97 [4].
Both original mutations are single nucleotide deletions that lead to frame shift and premature translational stop [2].
Interestingly, it has been proposed that coupled amino acid changes will mostly occur in the same lineage or on the same branch of the phylogenetic tree [4], [5], [22] due to the strong positive selection pressure to mutate another site to compensate the original mutation.
Our findings successfully demonstrate that the aggregation of a mutant protein can be suppressed by mutating the residues significantly displaced upon the original mutation.
She and Gusella are currently looking for genes that interact with the original mutation.
Two cases (#7, 12) lost the original mutation and one of them (#7) acquired a new one instead (Figure 2b).
Alternatively, some mutations at separate genes open up a new biochemical pathway that circumvents the block of function caused by the original mutation.
