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Outbreaks of the illness historically were limited to Africa and India, where Aedes aegypti, the original mosquito vector described for chikungunya virus, thrived.
Within a year or two, none of the original mosquito's offspring would be able to transmit the infection.
*Represents the original mosquito suspension during virus isolation attempts.
These 2 strains were reisolated from the original mosquito pools with the same technique.
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In the original, the mosquito (without top hat or briefcase) gorges itself on an alcoholic, becoming so drunk in the end that it cannot fly away.
This assay was adapted for the mosquito from the original assay developed for Drosophila (Ja et al., 2007) For each trial, five mosquitoes were fasted with access to water for 24 hr, and placed in a polypropylene vial (#89092 742, VWR, Radnor, PA) with access to two 5-µl calibrated glass capillaries (#53432 706, VWR) containing 10% (w: v) sucrose with 5% (v: v) green McCormick brand food dye.
This higher IR would have remained undetected if only pooled mosquitoes were analyzed, even though our original pools consisted of only 10 individual mosquitoes.
The NA/WN02 genotype is believed to have become dominant in North America because of its ability to more efficiently disseminate in mosquitoes than the original NY99 virus genotype (6 – 8 ).
Table 1 shows the insemination rates in artificial-mating crosses between An. albitarsis from the laboratory colony, F1 mosquitoes of the original population (feral) and their reciprocal hybrids.
For each founder colony, fine-mapping was performed by genotyping all of the unpooled individual mosquitoes from the original infections from which the initial phenotype pools were constructed.
We agree that this is an important point and had included a preliminary analysis of sporozoite migration patterns after inoculation by mosquito bite in the original manuscript.
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