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The original extracts were prepared at 200 mg ml 1 and further diluted with serum-free cell culture medium to obtain a series of diluted extracts (100, 50, 25, 12.5 and 6.25 mg ml 1).
For AEDA, the concentrated aromatic extract (200 μL) of CCA was 1 2 diluted stepwise using dichloromethane as the solvent to obtain serial dilutions (1 2, 1 4, 1 8 and up to 1 64) of the original extracts.
Another method that allows high-throughput identification of PPIs in original extracts is shotgun proteomics [ 59].
The original extracts, plus two dilutions (1 2 and 1 5) were printed in triplicate (printing replicates).
The products were analyzed by GC/MS using the same temperature programme as for the original extracts.
Then, the cell cultures were exposed to either 200 μL original extracts of the test specimens or a medium as a negative control.
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Then, culture medium was swapped for either fresh medium (negative control) or Zn-textile extracts in medium (original extract 100%, extraction ratio 1 g:50 mL) and serial dilutions of the original extract (75% at 0.75 g:50 mL, 50% at 0.5 g:50 mL, 25% at 0.25 g:50 mL, and 10% at 0.1 g:50 mL).
The original extracted DNA of samples was directly used without any DNA purification and amplification.
The original extract was obtained at 200 mg akermanite/ml LG-DMEM and further diluted with LG-DMEM.
We developed a method based on this europium complex for the specific detection of original extracted DNA from E. cloacae.
The final extracts were denoted as 1/2, 1/4, 1/8, 1/16, and 1/32 extracts based on the concentrations of the original extract.
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