In consideration of the equivalent gravity compensator, a new deletion rule is introduced and incorporated in the set of the original deletion rules.
We suggest that for reporting purposes, it is acceptable to use original deletion names that is, del(GJB6-D13S1830).
Illegitimate recombination between these repeated regions may offer a mechanistic explanation of the original deletion event itself.
For short-lived mutants, we assessed the original deletion library strains (Fig. 6A, solid blue circles) and the prototroph back-crossed mutants (Fig. 6A, open blue circles).
In fact, the relationship between original deletion effect and average effect of secondary mutations was positive and non-significant (r = 0.486, P = 0.407).
Interestingly, those aptazyme constructs that showed ligand-dependent activity closely resembled the original deletion variants that showed magnesium-dependent recovery of splicing activity.
The 3′ barcode from the deletion strain being targeted should remain intact due to selection of the URA3 marker that sits between the direct repeats on the 3′ end of both the allele and the original deletion strain.
For long-lived mutants, we assessed the original deletion library strains (Fig. 6A, solid red circles), the prototroph back-crossed mutants (Fig. 6A, open red circles) as well as two deletion mutants that were independently reconstructed (Fig. 6A, black circles).
Another approach transformed the original deletion collection (along with haploid DAmP alleles of essential genes) with a plasmid containing all the wild-type biosynthetic genes (Mulleder et al. 2012).
Again, this analysis included the original deletion library strains (Fig. 6B, solid circles), prototroph back-crossed mutants (Fig. 6B, open circles), and three independently generated mutants (Fig. 6B, black circles).
