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Using single molecule force spectroscopy (SMFS), we have investigated the single chain elasticity of a series of dendronized copolymers, whose original backbone is styryl dendron and maleic anhydride.
The GIs can also be defined over a smoothed backbone, rather than the original backbone.
GIs over the original backbone are heavily influenced by the local geometry within SSEs.
Generally, different dimensions of the SGM vector over smoothed backbone are more independent than that over original backbone (Røgen, 2005).
Hence, by deduction, they can accommodate mutations to amino acid types for which no rotamers fit in the original backbone.
For agroinfiltration, the 35S-TuMV-GFP-NosT cassette was cut from its original backbone and ligated into pGreenII.
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With a given Rd.HMM most of the sequences emitted by the HMMER emit-module gave energy scores as poor as random sequences, when forced back into the original 3D backbone with ROSETTA design (not shown).
To demonstrate this in the context of our new algorithm, we first used the original, distorted backbone and Cβ conformations.
Differential scanning calorimetry showed lower glass transition temperatures for the comb polymers compared to the original linear backbones.
Major differences in the viscoelastic responses of the original linear backbones and the comb polymers were observed, which were explained in terms of arm retraction/relaxation leading to tube dilation.
Moreover, the topological properties of the original network and backbone are compared in detail.
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