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Consequently, a more complete understanding of the dynamics within multipartite mutualism communities is essential for understanding the origin, specificity, and stability of mutualisms.
Moreover, origin specificity can be achieved by manipulating levels of ORC and competitor DNA.
However, we show that origin specificity can be conferred by reducing ORC concentration and including competitor DNA during pre-RC assembly.
The prediction model with the four Dutch markers, when applied to the French strains, had a sensitivity (for being of clinical origin), specificity, NPV and PPV prediction of 78%, 54%, 70%and65%5%, respectively (see Table 1).
This experiment shows that replication of the plasmid lacking a functional origin was also ORC dependent and that reducing ORC concentration reduced overall replication, but did not confer origin specificity.
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The miR447 family appears to have acquired novel target specificity and lost sequence-of-origin specificity, while miR856 may have acquired dual-targeting specificity (Figure 6, Table 2, Table S3).
However, the origin and specificity of the CD4+C25+ population generated by this or other helminth infections have yet to be delineated.
The relatively recent origin of specificity in this step has important implications for both the evolution of ABA biosynthesis and action in land plants.
It is easy to understand a possible origin of specificity when comparing Gi/o coupled GPCRs (which activate GIRK) and Gq coupled GPCRs (which do not activate GIRK) (Lei et al., 2001; Cho et al., 2005).
Moreover, in the presence of salmon sperm DNA, Orc1/Cdc6 displays enhanced ATPase activity [ 119], indicating that trypanosome Orc1/Cdc6 ATPase might be responsible for defining origin-binding specificity, similar to yeast Cdc6 [ 120].
The origins of specificity leading to acetylenases and desaturases are not currently evident from comparisons at the primary sequence level, and residues promoting acetylenase activity have yet to be located.
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