These ChIP experiments revealed that, for all the three analyzed origins, origin DNA was enriched 2 5 times over control by immunoprecipitating chromatin with antibodies recognizing Rb and E2F1 (Figs. 1B and 1D for Lamin B2 and GMS-CSF origins respectively).
At each of these origins, origin recognition complex (ORC) binding was abolished by introducing a four-base-pair mutation in the ORC-binding site at the native chromosomal loci.
In Of Spirit, Derrida calls this kind of origin "origin-heterogeneous": the origin is heterogeneous immediately (Of Spirit, pp. 107-108).
Sam 2: SAM synthase 2 gene sequence, His 4 ORF: Histidine 4 open reading frame, pBR322 origin: Origin of replication of the plasmid pPIC9K, lac Z: β-galactosidase gene Z, cbs: Cystathionine-β synthase gene, pUC origin: pUC origin of replication, Zeocin: Zeocin resistance gene.
The frequency and amplitude of spontaneous subthreshold responses were analyzed using the software Origin (Origin Lab Corp. Wellesley Hills, MA, USA).
There was no evidence for origin-environment interaction in plasma carotenoid levels (non-significant term "BSM*origin(duplicate)"), but there was a significant effect of nest of origin ("origin(duplicate)") (Table 2).
Data were analyzed with Origin (Origin Lab, Northampton, MA, USA), Microsoft Excel and Prism (Graphpad) software.
Figures were prepared using the Origin (Origin Lab, Inc., Northampton, MA) or CorelDraw (Corel Corporation, Ottawa, Canada) software packages.
The extracted time traces were processed using Matlab (MathWorks, Natick, MA) and Origin (Origin Lab, Northampton, MA).
The Glucose Reduction and Atherosclerosis Continuing Evaluation substudy of ORIGIN (ORIGIN-GRACE) is an investigator-initiated, randomized, controlled, parallel-group study with a 2 × 2 factorial design.
Data analyses were performed off-line with the aid of clampfit (Axon, Molecular Devices) and Microcal Origin (Origin Lab Corporation) software.
