Details on the origin of specimens and DNA extraction methods are provided by [20].
Moreover, quality and origin of specimens can influence the sensitivity of RT-PCR– and antigen-based tests.
Hematoxylin-eosin stained sections were, then, examined by the pathologist participating in the study, who was completely aware of the origin of specimens.
Patients records were screened for gender, age, potential mechanism of bacterial access to the bone, time of evolution of COM, bone involved, surgical access to take the bone specimen, origin of specimens cultured, genera and species of organisms identified, and antibiograms (Vitek Systems®, bioMerieux, Hazelwood, MO, USA).
The increase of HSV detection rates by PCR varied with the origin of specimen and was particularly significant for skin specimens (7/14 versus 3/14 detected by culture) and bronchoalveolar lavages (8/8 versus 1/8).
Isolates for identification are forwarded to MDL by public health laboratories with standardized forms that include information on clinical condition or suspected disease, date of onset, a brief case history, antimicrobial therapy, origin of specimen, and laboratory results.
Table 1 [see Additional file 1] lists specimen information, geographic origin of the specimens, names of collectors, and GenBank accession numbers.
In general, limited availability of samples of known origin, or reversely the lack of the information on the true origin of available specimens, constitutes the main drawback of the proposed approach.
Bioaccumulated metals or radioactive fission decay products are like chemical signatures: chemical and radiochemical analysis can be used to identify the origin of imported specimens, and for long-term radioecological monitoring of polluted areas.
The subgroups within this clade did not appear to correlate with the origin of the specimens from certain geographical areas (Fig. 1, File S2).
The cytb topology reflected the geographic origin of the specimens, and the five main evolutionary lineages of Fink et al. [33] and Heckel et al. [34] were found: W (France, Germany, Switzerland, Belgium, Spain), F (Germany), I (Switzerland, Italy), E (Germany, Austria, Hungary, Slovakia, Czech Republic, Poland, Ukraine, Russia, Finland) and C (France, Germany, Switzerland, Netherlands, Denmark).
