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This was deliberately performed without assumption as to the origin of sequence.
DNA extracted from buccal swabs (Quick-gDNAMiniPrep; Zymo Research) was used to detect the germline/somatic origin of sequence variations.
Furthermore, the use of paired end reads can facilitate fine-grained inference of the origin of sequence variants commonly observed in both CYP2D6 and CYP2D7.
Over the last five decades, the molecular clock has been central to debates in evolutionary biology (i.e., selectionism vs. neutralism), and provided a basis for estimating the divergence time of populations and species, detecting natural selection at genomic scales and understanding the origin of sequence variations (Rannala and Yang 2003; Kumar 2005; Yang and Rannala 2012; Du et al. 2013).
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The origin of sequences is difficult to decipher and remains somewhat uncertain.
The nematode origin of sequences was first investigated by a blastx search against the non- redundant nucleotide database in Genbank.
We sought to develop algorithms for examination of viral metagenomes to identify the origin of sequences independent of BLAST algorithms.
A robust diagnosis of sequence variation in the vicinity of the target SNPs will depend largely on sequence coverage, sequence quality [ 34] and origin of sequences as far as the number and relatedness of individuals surveyed for SNP discovery.
The genetic origin of sequences encoding the heavy and light chain variable (VL) domains, and mutational status of the transcripts was determined using the IMGT/V-QUEST web tool (program version 3.2.20; reference directory release: 201135-3) 25.
Previous studies, including ours, have revealed frequencies of gene transfer events, the origins of sequence elements, and a few possible mechanisms involved [ 5].
All satellites are encapsidated by the helper virus coat protein, and share an origin of replication sequence, the conserved nonanucleotide sequence, with their helper virus.
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