Last but not least, it may allow the discovery of the presence and parental origin of copy-neutral uniparental disomies, as well as of, for example, meiotic trisomies.
For the PGD-derived hESC lines, the SNP genotyping data were used to determine the origin of the copy number alterations.
In contrast, our findings regarding the statistical distribution of DMs are novel and confirm a long held suspicion of the origin of oncogene copy-number heterogeneity.
This approach can resolve relationships among sequences with low diversity and can thus be used to infer the origin of multiple copies from a unique sequence.
In contrast there are no long branch copies of Cocv2 (supplementary fig. S4 A, Supplementary Material online) and the phylogeny has a star-like appearance, highlighting a recent common origin of all copies in the genome.
The fosmid vector contains both the single-copy Escherichia coli fertility factor origin, which enhances fosmid stability, and the multi-copy IncPα origin, allowing amplification of copy number upon induction with l-arabinose.
The plasmid contains a high copy ColE1 origin of replication (∼100 copies), the TetR repressor protein and an ampicillin resistance marker (Supplementary Figure S1a).
The interaction of region of origin with pfmdr1 copy number could not be investigated because of insufficient multicopy samples from Africa in the model.
In D. virilis, D. mojavensis and D. grimshawi, we detected a second origin of a retroposed copy, but in this case the parental copy is still preserved.
The use of lead antimonate (found in nature as the mineral bindheimite) to render the halo around Mary's head is consistent with a mid-18th to late-19th century date of origin of this smaller copy of the original Rubens painting.
This is consistent with a recent origin of these IS copies and the high copy numbers of recently expanded IS groups, which generates many pairwise comparisons with no or very low divergence.
