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After the fiber orientation was calculated using the algorithm described in Sec.
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Orientation selectivity was calculated using the previously described orientation selectivity indices [18].
Split support was calculated using SumTrees.
Statistical significance was calculated using R software.
Relative expression was calculated using ΔΔCt.
Nucleotide diversity was calculated using DNAsp v4 [71].
Each statistic was calculated using 10,000 simulations.
Object orientation can be calculated using second-order moments.
Setting a computational routine, θ p and f can be determined, and the degree of molecular orientation can be calculated using the following equation [ 28, 29, 31, 32]: (6) ∫ γ = 0 γ = 2 π ∫ α = 0 α = 2 π ∫ β = 0 β = π P 2 (cos β ) f sin β d β d α d γ = 〈 P 2 (c o s β ) 〉, where 〈 P2(cos β)〉 is referred to as Herman's orientation parameter and represents the degree of molecular orientation.
These orientations can be calculated using the multiple orientation framework in [17].
The strain energy is evaluated by numerical integration involving the fiber orientation angle, and is calculated using the derivative of the spline function in minute intervals.
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