The cartilage thickness was then measured at an exact location and orientation site with a penetrating steel needle probe.
The distribution, morphology, number, orientation, and sites of origin and insertion of the meningo-vertebral ligaments were observed.
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Testing the virtual reality (VR) models in three-dimensional (3D) spatial design showed that 2D models and aerial photos are also needed to support orientation on-site, and the best results are achieved by combining these tools together.
Another important issue is that without special geometric constraints, passive 3D looping will not preserve relative site orientation and will also allow communication between sites on separate DNA strands [ 23– 25].
For cooperatively acting transcription factors though, there could be variation in the orientation of the sites relative to each other.
Ectopic recombination requires the presence in the parental chromosome of two homologous TE copies inserted in opposite orientation at different sites.
However, further analysis on five putatively interacting TF pairs suggests that such interactions may be relatively insensitive to the distance and orientation of binding sites.
pCGT7-SF2/ASF-antisense expression plasmid was created with the following primers: SF2 -F: 5'-ACCTTCCAGGATCCATGTCGGGAGGTGGTGTGATT-3 and SF2-R: 5'-ACCTTCCATCTAGATTATGTACGAGAGCGAGATCT-3', and was cloned into the pCGT7 vector in reverse orientation at XbaI/BamH1 sites.
However, there is a bias in the orientation YY1 binding sites in OLFR genes.
