Most metals and alloys in usable form display an internal microstructure characterized by a collection of grains with different lattice orientation separated by grain boundaries.
Scanning electron microscopy using electron-backscattered diffraction reveals the formation of near periodic patterns comprising domains with alternating orientation separated by narrow boundaries.
The complete RNAi vector containing MIPS (in both sense and antisense orientation, separated by wheat RGA2 intron) under the control of Ole18 promoter (pOle18-MIPS-006) was used for rice transformation experiments.
The central part of the grain contains broad areas with relatively consistent orientation separated by a small number of discrete linear zones that transect the short axis of the grain and which accommodate 1 5° misorientations (Fig. 2c,d).
Calculations show that the most energetically favorable island configurations are single dimer on a row in non-epitaxial orientation [43, 45, 48, 49] (Figure 5b), complexes of two dimers (pairs of dimers) in epiorientation (metastable [43]) and two dimers on a row in non-epitaxial orientation separated by a divacancy, and tripple-dimer epi-islands considered as critical epinuclei [43].
The silencing construct (pEC129) was derived from pWBVec8 and contained two copies of a 575 bp fragment of the PviCAD2 coding region in inverted orientation separated by a spacer region derived from the barley Cre intron.
An analysis of the PGS nucleotide sequence of KT71T revealed a total of 15 putative PpsR binding sites, of which 12 were arranged in a typical tandem orientation separated by seven or eight nucleotides (Figure S5).
These 29-mers were incorporated in the forward and in the reverse orientation, separated by a TCAAGAG loop, in the vector pRS shRNA by the manufacturer to generate four pRS-based gene-specific rat ZnT8 shRNA expression vectors (HuSH; Origene).
L4440 (pPD129.36) contains two convergent T7 polymerase promoters in opposite orientation separated by a multicloning site [ 21].
The L4440 (pPD129.36) vector contains two convergent T7 polymerase promoters in opposite orientation separated by a multiple cloning site [ 25].
