Sentence examples for orientation of the insert from inspiring English sources

RNA hybridization probes were generated from late tailbud derived cDNA by subcloning the coding regions of the genes of interest into the pCRII-TOPO dual-promoter cloning vector (Invitrogen, KH461020) and PCR was performed to confirm the orientation of the insert.

Correct orientation of the insert was determined by DNA sequencing.

Correct orientation of the insert was confirmed by sequencing.

All clones were screened for the correct orientation of the insert and were checked by sequencing.

The clone was checked for correct orientation of the insert by restriction enzyme digestion and confirmed by sequencing.

Correct orientation of the insert was confirmed by restriction enzyme mapping and sequencing with BigDye 3.1 (Applied Biosciences, Carlsbad, CA) according to the manufacturer's protocol and analyzed on an ABI PRISM 3700 DNA Analyzer (Applied Biosciences).

All substrates were sequenced to verify insertion and orientation of the inserts.

For each trap line, tissue PCR [ 113] was performed to confirm the insertion site and determine the orientation of the inserted element by using a gene-specific primer and a uidA specific primer.

The location, size, and orientation of the inserted lambda DNA segment was verified by restriction endonuclease digestions and by heteroduplex analysis.

The orientation of the inserts was verified by ScaI analytical digestion.

First, it provides an anchor for primers when these are used to check the orientation of the inserted transgenes.